Journal: Science Advances
Article Title: SARS-CoV-2 receptor binding domain displayed on HBsAg virus–like particles elicits protective immunity in macaques
doi: 10.1126/sciadv.abl6015
Figure Lengend Snippet: ( A ) Design of the nonhuman primate study in cynomolgus macaques. ( B ) Titers of RBD-specific antibody binding in animal sera. ( C ) Titers of SARS-CoV-2 pseudovirus–neutralizing antibody in animal sera. NT 50 , end point of 50% reduction of virus expression. ( D ) Expression of IFN-γ from cells stimulated with S1 protein peptides. Statistical significance was determined by a Kolmogorov-Smirnov test. n.s., not significant ( P > 0.1). Black bars represent median values. Gray dotted line represents limit of detection. PBMCs, peripheral blood mononuclear cells.
Article Snippet: Plates were then washed with wash buffer (MilliQ water with DPBS and Tween 20) and incubated for 2 hours at room temperature with polyclonal rabbit anti-human IFN-γ biotin from U-Cytech at 1 μg/ml.
Techniques: Binding Assay, Expressing